Part:BBa_K879222

Blue with promoter 2

This is the expression cassette that we built containing blue pigment protein amilCP, coupled with the relatively weak promoter 2, and the new RBS engineered for amilCP . To the naked eye, it appears blue. Under UV light, there is no fluorescent colour.

Usage and Biology

Colour quality. The amilcp (blue) colour was used as reporter in this experiment as it only takes a few seconds to analyze. The above figure shows the blue color expression of this colour cassettes under the UV light, to the naked eye, and overnight culture. Note that amilCP is not a fluorecent chromophore- too bad!

The color intensity using the new engineered RBS is excellent, comparable to the original Uppsala part with the B0034 RBS under control of Bba_J23101, a much stronger promoter than promoter 2. This could be useful as a reporter in contexts of lower transcriptional levels. Additionally, since the regulatory sequences are different from the other color parts BBa_K879311 and BBba_K879313, the risk of recombination will be minimized when multiple colors are used in the same cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 43
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1